Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

Polovinkin, V.; Khakurel, K.; Babiak, M.; Angelov, B.; Schneider, B.; Dohnalek, J.; Andreasson, J.; Hajdu, J.

doi:10.1107/S1600576720013096

Journal of Applied Crystallography Journal of Applied
Crystallography

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Figure 2
Images of bacteriorhodopsin crystals before cryo-FIB milling. Bright-field optical micrograph (a) of BR crystals grown in an LCP of monoolein at room temperature. SEM (b) and FIB (c) micrographs of a flash-frozen BR crystal with leftovers of the crystallization medium. (d) Geometrical features of the hexagon-shaped BR crystal are indicated by blue dashed line on the FIB image. These features can be guessed by comparing (a), (b) and (c). The red-dashed-line rectangle in (d) shows the selected area for further cryo-FIB milling. The scale bars correspond to 50 µm.

JOURNAL OF
APPLIED
CRYSTALLOGRAPHY

ISSN: 1600-5767

Volume 53| Part 6| December 2020| Pages 1416-1424

https://doi.org/10.1107/S1600576720013096

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