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Figure 10
CHROMIXS updates. (a) Regions in the SEC-SAS data (blue line) which represent the sample (green, on the peak) and buffer (red, on the flat region) can be selected manually or automatically. The Rg or MW across the sample region (black correlation, through the sample elution peak) can be calculated. (b) Complementary time-course data (black dots), such as a UV absorbance trace to track protein elution, can be loaded and viewed together with the SEC-SAS data. The third (rightmost) UV absorbance peak corresponds to buffer mismatch, i.e. components in the sample buffer that are not present in the SEC running buffer.

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 54| Part 1| February 2021| Pages 343-355
https://doi.org/10.1107/S1600576720013412
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