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Figure 9
Number of tilts Nθ,A of equation (15)[link] required for complete coverage of information in the Fourier transform representation of the specimen. Values of Nθ,A are shown for δr = 20 nm-resolution imaging of copper features in silicon (a) and protein features against ice with an overall thickness of tissue consisting of 30% protein/70% ice (b). The number of tilts Nθ,A is smaller than what would be required to meet the Crowther criterion Nθ [equation (7)[link]] when reconstructing NA axial planes in beyond-depth-of-field imaging (Jacobsen, 2018BB67). For each sample thickness t, the photon energy Et that minimizes the per-pixel imaging time Tp was used in calculating Nθ,A; this photon energy Et is shown in Fig. 7[link].

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