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Figure 5
DPP8 crystal stability in different soaking solutions. (a)–(d) A DPP8 crystal is transferred sequentially to new drops with increasing concentrations of TMAO and DMSO, reaching up to 3 M and 5%, respectively. (e), (f) Classical soaking of a DPP8 crystal at 4°C. 1G244 was dissolved to 1 mM from a stock solution of 100 mM (precipitated 1G244 was readily visible). After 5 min the crystal cracked and then dissolved. (g), (h) Classical soaking of DPP8 crystals with 1 mM EIL peptide at 20°C. After 5 min the crystal cracked and then dissolved. All conditions had 0.46 M Na citrate as a base component. (i)–(l) DPP8 crystal mounted in the humidified air stream, before (native) and after spraying of 1G244 (j) and the EIL peptide (l) with the aerosol composition noted accordingly. In contrast to the classical soak experiments the crystals showed no visible degradation. Crystals showed long-term stability if time is not mentioned.

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 54| Part 3| June 2021| Pages 895-902
https://doi.org/10.1107/S1600576721003551
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