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Figure 1
HiLiDe crystallization of MhsT. (a) Crystallization phase diagram. The curve shows the boundary between the precipitation and crystallization zones as a function of the amounts of lipid (x axis) and detergent (y axis) that were added to a fixed amount of protein in the HiLiDe relipidation procedure (p/l/d ratio = 3/x/y). Dashes indicate that there are no data points above this part of the curve. Relative crystal sizes are indicated in the diagram, and letters refer to (b). Crystals subjected to X-ray diffraction tests are coloured – green refers to cases where data sets could be processed and blue refers to cases where diffraction quality was too poor to enable processing (see the main text). Resolution annotations refer to the highest resolution at which diffraction spots could be observed (allowing comparison of resolution potential of all tested crystals, whether or not data sets could be processed). (b) Representative crystal images. The l/d ratios used to obtain the crystals shown here are given in (a).

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