Figure 2
HiLiDe crystallization of SERCA1a. (a) Crystallization behaviour as a function of the amount of detergent used for solubilizing the protein from native membranes. The amount of detergent added to the protein is given on the axis, and the resulting protein concentration and relative sizes of the crystals are indicated. (b) Representative crystal images. The p/d ratios used to obtain the crystals shown here are given in (a). The scale bar in `e' applies to all the images. (c) X-ray diffraction tests. Data sets were collected on two or three crystals for each tested p/d ratio, and the maximum resolutions of the processed data sets were plotted (blue, the crystals showing highest resolution; orange, the crystals showing lowest resolution; grey, the crystals showing intermediate resolution in cases where three were tested). |