Figure 4
NS-TEM images of an MhsT crystal and putative precursors. (a) Images of an MhsT crystal recorded at different magnification levels. The inserted box is three protein spacings long in each direction. (b) Crystal packing of a previously determined MhsT structure (PDB ID 4us3). A single molecule is shown along with the unit cell (top). Three-by-three molecules in the ab plane, coloured differently for different membrane layers, are also shown (bottom). The box around them has the same dimensions as the box in (a). (c) Images of a lamellar structure (that may be a crystal precursor) recorded at different magnification levels. Scale and orientation are the same as in (a). (d) Images of a hybrid structure recorded at different magnification levels. The numbers mark three morphologically different zones, and the dashed box on the left corresponds to the region shown on the right. |