Co-flow injection for serial crystallography at X-ray free-electron lasers

Doppler, D.; Rabbani, M.T.; Letrun, R.; Cruz Villarreal, J.; Kim, D.H.; Gandhi, S.; Egatz-Gomez, A.; Sonker, M.; Chen, J.; Koua, F.H.M.; Yang, J.; Youssef, M.; Mazalova, V.; Bajt, S.; Shelby, M.L.; Coleman, M.A.; Wiedorn, M.O.; Knoska, J.; Schön, S.; Sato, T.; Hunter, M.S.; Hosseinizadeh, A.; Kuptiz, C.; Nazari, R.; Alvarez, R.C.; Karpos, K.; Zaare, S.; Dobson, Z.; Discianno, E.; Zhang, S.; Zook, J.D.; Bielecki, J.; de Wijn, R.; Round, A.R.; Vagovic, P.; Kloos, M.; Vakili, M.; Ketawala, G.K.; Stander, N.E.; Olson, T.L.; Morin, K.; Mondal, J.; Nguyen, J.; Meza-Aguilar, J.D.; Kodis, G.; Vaiana, S.; Martin-Garcia, J.M.; Mariani, V.; Schwander, P.; Schmidt, M.; Messerschmidt, M.; Ourmazd, A.; Zatsepin, N.; Weierstall, U.; Bruce, B.D.; Mancuso, A.P.; Grant, T.; Barty, A.; Chapman, H.N.; Frank, M.; Fromme, R.; Spence, J.C.H.; Botha, S.; Fromme, P.; Kirian, R.A.; Ros, A.

doi:10.1107/S1600576721011079

Figure 1
(a) The experimental setup of the co-flow generator at the EuXFEL (not to scale). (1) Flow-rate sensors, (2) crystal suspension reservoir mounted in the rotating anti-settler device, (3) oil reservoir, (4) nozzle rod and (5) hybrid device (3D-printed device with integrated co-flow generator and GDVN) mounted on the end of the nozzle rod. Black lines indicate capillary tubing or fused silica capillaries for fluid and gas transport. (b) The assembled hybrid device mounted in the nozzle rod. The co-flow/nozzle hybrid is located at the very end of the one gas and two fluidic lines connected to the fused silica capillaries through epoxy. (c) An image of the assembled Y-junction device. Co-flow is generated as indicated by the interface between the two phases (see yellow arrow). (d) A schematic drawing of the Y-junction device for co-flow formation designed in Fusion 360. (e) An image of the T-junction hybrid device, showing the oil–sample co-flow running (marked with a white arrow) and the jet leaving the nozzle. (f) A schematic drawing of the T-junction device for co-flow formation designed in Fusion 360. The scale bars represent 200 µm in panels (c)–(f).

JOURNAL OF
APPLIED
CRYSTALLOGRAPHY

ISSN: 1600-5767

Volume 55| Part 1| February 2022| Pages 1-13

https://doi.org/10.1107/S1600576721011079

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