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![[Figure 2]](ap5042fig2mag.jpg)
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Figure 2
Dehydrating effects on crystallization drops from significantly different conditions after 24 h from adding KF13 to the reservoirs. Initial drop volume was 200 nl of reservoir plus 200 nl of a protein-less solution containing 500 mM NaCl. The conditions are from a crystallization plate from our facility and contain 2.5 M ammonium sulfate, 0.2 M NaCl and 0.1 M MES pH 5.6 (A10); 1.2 M ammonium sulfate and 0.1 M MES pH 5.9 (A11); 14% ethanol and 0.1 M ADA pH 6 (B8); 18% ethanol and 0.1 M bis-tris propane pH 7.1 (B9); 15% PEG 2K (w/v) and 0.1 M bis-tris propane pH 6.9 (C10); 8% PEG 20K, 8% PEG 2K (w/v), 0.25 M KBr and 0.1 M sodium acetate pH 4.5 (C11); 21% PEG 3350, 0.15 M NaCl and 0.1 M MES pH 6 (D6); 15% PEG 3350 and 0.1 M MES pH 6.2 (D7); 28% PEG 400, 0.2 M NaCl and 0.1 M MOPS pH 6.5 (E7); 25% PEG 400, 4.5% ethanol, 1.5 mM MgCl2 and 0.07 M MES pH 6.6 (E8); 14% PEG 4K, 6% MPD and 0.1 M sodium potassium phosphate pH 6.2 (F4); 29% PEG 4K, 0.1 M ammonium sulfate, 0.1 M magnesium acetate and 0.1 M sodium citrate pH 6.5 (F5); 13% PEG 8K, 0.09 M ammonium sulfate and 0.05 sodium cacodylate pH 6.5 (G8); 20% PEG 8K, 0.2 M magnesium acetate and 0.1 M MES pH 6.5 (G9); 1 M potassium phosphate monobasic, 3% isopropanol and 0.1 M sodium cacodylate pH 6.5 (H1); 1.4 M sodium acetate and 0.1 M sodium cacodylate pH 6.5 (H2). |
![[Figure 2]](ap5042fig2.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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