Figure 3
Reconstruction from simulated SPI data sets. SPI data sets from a chaperonin were simulated in the absence or presence of noise. A multi-tiered iterative phasing algorithm was used to recover the protein structure from 5000 images of the indicated data set. (a) Isosurfaces of the density map reveal the loss of eightfold symmetry with increasing noise. The resolution of each reconstruction is noted in parentheses. (b) The resolution was measured as the spatial frequency at which the FSC between the reconstructed and reference maps dropped to 0.5. |