Figure 1
Overall design of the 3D-printed flow cell. (a) Flow cell with a 3D-printed base glued to an X-ray transparent glass (or quartz) capillary containing a channel through which a fused silica capillary tube is inserted and through which the sample is transported. This device is supported by a magnetic connection (either an iron or magnetized bullet) held by friction. The dimensions of the base can be adjusted to be compatible with any goniometer magnet used at any synchrotron radiation source. (b) Flow cell device connected to a syringe pump using suitable connectors. (c) Schematic of how the flow cell is used as a sample delivery system at a macromolecular crystallography beamline. Microcrystals (red) are injected from a syringe connected to a pump and delivered into an X-ray beam through an X-ray transparent capillary held in place on a standard goniometer magnet. X-ray diffraction data from microcrystals are collected on a rapid-readout X-ray detector. |