Figure 5
Equilibration at 4°C of 10%(w/v) reservoir solutions of ammonium sulfate with capillary tubes of 4.9 cm length oriented in the vertical direction as a function of equilibration time. An 8–10 kDa MWCO membrane separates the capillary and reservoir solutions. Symbols are averages from experimental measurements and the error bars represent one standard deviation for no gel plug (diamonds) and a 1 cm 3% agarose gel plug (circles). Lines represent the percentage equilibration modeled for diffusive transport of precipitant from the reservoir to the capillary. |