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Figure 5
SEC–SANS data example for a 50 kDa protein–DNA complex protonated in D2O buffer. The complex concentration was approximately 2.5 mg ml−1 and the exposure time was 10 + 4 h. Data were reduced using GRASP, buffer subtracted, merged and concentration normalized using SANS reduction macros written by S. Kline for the Igor Pro software, and plotted using Igor Pro. |
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