Revealing cholesterol effects on PEGylated HSPC liposomes using AF4–MALS and simultaneous small- and wide-angle X-ray scattering

Hsu, T.-W.; Yang, C.-H.; Su, C.-J.; Huang, Y.-T.; Yeh, Y.-Q.; Liao, K.-F.; Lin, T.-C.; Shih, O.; Lee, M.-T.; Su, A.-C.; Jeng, U.-S.

doi:10.1107/S1600576723005393

Journal of Applied Crystallography Journal of Applied
Crystallography

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Figure 2
(a) Evolution of the liposome concentration, particle mass, R_g and R_h measured over the AF4 flow of the sample solution of PEGylated HSPC liposome with cholesterol, with R_g and R_h deduced from the MALS and DLS data, respectively. The mass was deduced from the combined analysis of MALS and dRI data. (b) Derived number-density distribution of the liposome as a function of R_h, fitted with a Gaussian profile (solid curve). (c) R_g versus R_h presentation. Data are fitted with a solid line (slope R_g/R_h = 1.05). Also shown is a red dashed line (slope = 1.0) representing the shape factor of ideal shells with R_g/R_h = 1.

JOURNAL OF
APPLIED
CRYSTALLOGRAPHY

ISSN: 1600-5767

Volume 56| Part 4| August 2023| Pages 988-993

https://doi.org/10.1107/S1600576723005393

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