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Figure 1
Schematic representation of the VIALS workflow. (a) Flowchart depicting the semi-automated high-throughput procedure of VIALS. Step 1: design and automated preparation of a rational grid screen based on a previously known crystallization condition (e.g. for the growth of large crystals). Step 2: tiny amounts of protein-laden LCP string are manually injected into each previously prepared small glass vial. Step 3: periodic direct inspection of crystal growth using a stereo high-magnification microscope. Steps 4–5: scale-up process once the optimal microcrystal size/density condition is found. Step 6: microcrystal growth and storage for SX measurements. (b) VIALS experimental setup for ligand soaking in meso in parallel ahead of room-temperature SX data collection.

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 56| Part 5| October 2023| Pages 1361-1370
https://doi.org/10.1107/S1600576723006428
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