Figure 3
Fluorescence confocal optical sectioning of H-target-containing cells. Fluorescence signal is produced by the co-expressed cytosolic YFP, whereas target H crystals are identified by the absence of signal. A Z value of 0 corresponds to the bottom of the cell. The optical sectioning (2.4 µm steps) confirms the relatively random orientation and position of the crystals throughout the cell body, as well as the presence of clustered, multi-shaped crystals. All values correspond to micrometre units. |