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![[Figure 3]](jo5110fig3mag.jpg)
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Figure 3
A schematic drawing of a typical counter-diffusion setup with a 1″ × 1″ microfluidic chip. The figures are exaggerated near the inlets compared with the real dimensions. For (a) sample loading, the protein solution (yellow) is loaded into the microfluidic channel using a pipette, the adhesive spacer (green) creates the sample flow layer in between the two pieces of thin film (dark blue) and the acrylic frame (gray) prevents the thin film from collapsing. For (b) crystallization, after loading, the chip is rotated upside down with open inlets contacting the precipitating buffer (light blue), and the setup is contained in a storage chamber to prevent dehydration. (c) When the crystals are fully grown, the precipitating buffer is removed and the chip is sealed with Crystal Clear tape. Before exposure to X-rays, all tape above the thin-film windows is removed, leaving only inlets sealed to prevent dehydration during data collection. |
![[Figure 3]](jo5110fig3.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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