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Figure 3
Mesh scans showing the variation of crystal quality over the supersaturation gradient within a capillary. (a) Photograph showing the 0.2 mm capillary with 60 mg mL−1 protein concentration from the S1 screen under cross polarized light after sealing and before data collection. The dashed lines indicate approximately the regions used for the mesh scan, with the bottom end of the capillary shown on the left at the site of precipitant entry. (b) The mesh scans are plotted as total integrated signal over the defined area (X, Y) with the relative intensity signal scale bar on the right and the N scores calculated for each area indicated above. (c) Diffraction pattern corresponding to the mesh scans above. The red and green box regions show enlarged portions of the diffraction images to emphasize differences in the quality of diffraction spots (d spacing corresponding to the corner of the diffraction images is 1.25 Å).

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APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
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