Figure 1
SANS pattern of streptavidin protein (0.5 mg ml−1) in buffer solution (Houston et al., 2018 ). The green and black solid symbols and line represent the experimental scattering patterns of the protein solution and buffer, respectively, recorded at detection distances of LD = 4 m (black) and LD = 1.5 m (green) with λ = 5 Å over an acquisition time of 1800 s in each configuration. The open blue symbols show the protein scattering pattern after correction of the buffer contribution. The red curves show the calculated scattering pattern based on the respective PDB structure (PDB code 1n7y; Le Trong et al., 2003 ) using the computational code PEPSI-SANS, ILL Grenoble (Grudinin et al., 2017 ): with (solid) and without (dashed) consideration of the incoherent background. |