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Figure 4
Visualization of missing parts through ER. The same models as in the previous figures, this time as a set of 25 ER models, coloured from N- to C-termini. In the top row, the four NCS-related copies show that the SRL (yellow loop) can extend in a highly disordered manner into a solvent void, in a similar fashion for each protomer. In contrast, the conformation of the termini is different in each of the four copies due to the different packing contacts and available void space. In the T1 apo structure models (bottom left), the termini assume a distinct, packing-induced secondary helical structure, while the SRL now folds back covering the entrance to the empty substrate-binding site. Even for the two atomic-resolution structure models (bottom right) where the absolute B-factor scale coloured models in Fig. 2[link] suggested a rigid N-terminal helix, the dynamic nature of this extension becomes evident in the ensemble models.

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 58| Part 2| April 2025| Pages 615-621
https://doi.org/10.1107/S160057672500130X
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