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Figure 3
Separation resolution at the SANS cell. (a) SEC chart for the unpurified BSA solution measured using a UV–Vis–NIR unit at a wavelength of 280 nm. Red, yellow and blue arrows represent the elution volume ranges at which the eluted samples were loaded into the SANS cell. The elution volumes of each fraction were (1) 13.8–14.1 mL, (2) 14.0–14.3 mL and (3) 14.2–14.5 mL. (b) Red, yellow and blue lines indicate the AUC results for fractions #1, #2 and #3, respectively. The black line shows the AUC result for monomeric BSA immediately after standard SEC purification with the same column. The molecular mass values calculated from the sedimentation coefficient and peak area ratio for each peak are included in (b). |