Figure 4
SANS profiles of (a) OVA, (b) BSA and (c) AF. Blue and red circles in the upper panels show the concentration-normalized SANS profiles obtained using SEC–SANS and AUC–SANS, respectively. Solid green lines indicate the calculated scattering profile from the crystal structures [PDB entries 1ova (Stein et al., 1991 ), 4f5s (Bujacz, 2012 ) and 4v1w (Russo & Passmore (2014 ) for OVA, BSA and AF, respectively]. Insets represent their Guinier plots. Solid blue and solid red lines in the Guinier plots indicate least-squares fitting using the Guinier formula for SEC–SANS and AUC–SANS results, respectively. Lower panels represent the ratio (r) of SEC–SANS to AUC–SANS scattering profiles. |