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![[Figure 3]](ju5087fig3mag.jpg)
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Figure 3
Radially averaged FFTs of TEM images of thylakoids in Symbiodinium (Slavov et al., 2016  ). (a) 24°C and dark-adapted, (b) 31°C and light-irradiated. The magnitude of the k vector (inverse distance) on the x axis of each power spectrum has been scaled to equivalent q (= k/2π) for ease of comparison with the SANS spectra obtained here. Clear maxima labelled B are assigned to the first- and higher-order repeat distances (RD) of thylakoid triplets. The respective RD values are (a) 556 ± 82 Å and (b) 537 ± 40 Å. The estimated uncertainty of the RDs is taken from the half-width at half-height on the lower-q side of the respective first-order peak. These maxima are consistent with the thylakoid RDs measured directly from the image. The peaks labelled F are 2D image equivalents of form factor peaks in a 3D SANS experiment. These correspond to the sub-structure inside the 2D projection of repeating triplets in the real-space images. They arise from unknown grey-level image contrast functions, in this case set by selective interaction of the thylakoid components with the OsO4 and uranyl acetate employed by Slavov et al. (2016) in enhancing their image contrast. Since the contrast functions are not known, we do not use these any further here. |
![[Figure 3]](ju5087fig3.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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