Figure 7
values neglecting the effects of membrane tension (i.e. assuming ) for POPC vesicles containing a mole faction of 10% POPS in different osmotic stress conditions. The vesicles were hydrated and extruded in 20 mmol L−1 (mM) sucrose in D2O and then diluted with either 20 mM sucrose or a more concentrated sucrose solution to induce an osmotic pressure gradient across the membrane. The data were fitted using a viscosity of 0.00111 Pa s for the 20 mM sucrose solution and 0.00116 Pa s for the 60 mM sucrose solution. Error bars represent one standard deviation from the least-squares fit to the NSE data. |