Determination of water permeability in stress-free lipid membranes through time-resolved small-angle neutron scattering

Frewein, M.P.K.; Perez-Salas, U.; Ollila, O.H.S.; Pabst, G.; Porcar, L.

doi:10.1107/S1600576726007788

Figure 1
Permeability measurements by TR-SANS. (a) Schematic drawing of the experimental setup: D ₂O exchange buffer and lipid vesicles in H ₂O are injected simultaneously and irradiated by a neutron beam (n). The simulated intensity decay at low q illustrates how the SLD in the vesicle core (yellow) gradually matches the surrounding solvent (orange) due to permeation. (b) Stopped-flow device mounted on the D22 SANS instrument, featuring a custom-designed small-volume head with temperature control. A nitrogen stream prevents condensation during measurements at low temperatures.

JOURNAL OF
APPLIED
CRYSTALLOGRAPHY

ISSN: 1600-5767

Volume 59| Part 5| October 2026| Pages 1550-1556

https://doi.org/10.1107/S1600576726007788

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