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Figure 2
Small-angle X-ray scattering (SAXS) of PPD1. (a) The experimental spectrum is shown with a simulated fit obtained using GASBOR. The radius of gyration, Rg, was estimated from the low-angle scattering region using a Guinier plot, i.e. log(intensity) versus q2. The distance-distribution function, P(r), with maximum linear dimension Dmax = 159.4 Å, is shown in the insert. (b) The ab initio shape reconstruction by GASBOR using P32 symmetry, showing agreement between the predicted molecular shape (grey beads) and the hexameric construct based on the crystal structure (black cartoon). Water molecules have been omitted.

Volume 1| Part 2| February 2014| Pages 101-109
ISSN: 2052-2525