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Figure 2
Protein crystallization in emulsion droplets stabilized by surfactant. Ideal drop sizes were first identified using polydisperse emulsion droplets. Monodisperse emulsions were used to produce identical crystals for diffraction experiments. Droplets were stored in a rectangular glass capillary. (a)–(c) Polydisperse emulsions of (a) D1D2 heterodimer from human spliceosomal snRNP particle, (b) concanavelin A and (c) trypsin. (d) Protein and precipitant solutions were introduced in a co-flow geometry under laminar flow conditions that prevent mixing upstream of the nozzle where both solutions became encapsulated into emulsion droplets. (e), (f) Monodisperse emulsions of (e) glucose isomerase and (f) lysozyme crystals. See main text for crystallization conditions. (d) and (f) are from Akella (2014BB2).

IUCrJ
Volume 1| Part 5| August 2014| Pages 349-360
ISSN: 2052-2525
doi:10.1107/S2052252514016960