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Figure 4
(a) Monodisperse emulsions were prepared using a dedicated dropmaking chip as illustrated in Fig. 2[link](d) and directly routed into the chip for serial crystallography for storage. (b) We used a laser-cut frame to hold and to port into the X-ray semi-transparent chip. (c) The X-ray semi-transparent chip mounted on the goniometer inside the Cornell CHESS F1 beamline. (d) Glucose isomerase crystals inside of the microfluidic device. Using a motorized stage, each crystal can be centered in the collimated X-ray beam. The beam is 100 µm in diameter. (e) A representative diffraction pattern of a glucose isomerase crystal taken at room temperature from inside the chip. Crystals diffracted to 1.4 Å resolution with a mosaicity as low as 0.04°. The bottom-right quadrant shows the diffraction pattern after background subtraction, using the Adxv diffraction pattern visualization tool (https://www.scripps.edu/~arvai/adxv.html ) with subtract background option.

IUCrJ
Volume 1| Part 5| August 2014| Pages 349-360
ISSN: 2052-2525
doi:10.1107/S2052252514016960