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Figure 6
(a), (b) Protein concentration as a function of distance from a simulation of nucleation and growth in one dimension. The concentration is dimensionless. The red dotted line indicates the initial concentration with a supersaturation of 83.3 at t = 0. The sites with concentrations that exceed the red line are in the crystalline phase, while those below are in solution. (a) Concentration profile at t = 250. Slow nucleation rate of R = 1 in dimensionless units. (b) Concentration profile at t = 50. Fast nucleation rate of R = 27. (c) The average number of crystals per drop as a function of time, [\langle x(t)\rangle], for two nucleation rates obtained from simulation and fitted to equation (3)[link], [{\langle x(t)\rangle=x_{{\infty}}[1-\exp({{-kt}})]}]. The conditions are the same as in (a) and (b). (d), (e) Fitting parameters to equation (3)[link] as a function of drop size for two nucleation rates, R = 27 and R = 1. Arrows indicate the size of the depletion zone. (d) The solid lines are the simulated final number of crystals per drop, [{x_{{\infty}}}]. The dashed lines are equation (7)[link], [x_{{\infty}}=V/w^{d}]. (e) The solid lines are the simulated rate of crystal formation, k. The dashed lines are equation (8)[link], k = JV. (f) Conceptual schematic. A drop of volume V can be thought of as [{x_{{\infty}}}] smaller independent drops of volume [{w^{d}=V/x_{{\infty}}}]

IUCrJ
Volume 1| Part 5| August 2014| Pages 349-360
ISSN: 2052-2525
doi:10.1107/S2052252514016960