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Figure 7
CTBAAAAMPR resolved as an oligomer by SEC–HPLC. Pink line, the Talon column eluate (not concentrated). Blue line, 10× concentrated eluate sample. Red line, 20× concentrated eluate sample. Spectrograms were normalized to the highest peak. Inset: proteins in fractions corresponding to the main peak of the 20× concentrated eluate chromatogram were resolved by SDS–PAGE under nondenaturing (ND; no DTT and no boiling) and denaturing (D) conditions. Molecular-weight standards indicate that CTBAAAAMPR is organized into SDS-stable pentamers.

Volume 1| Part 5| August 2014| Pages 305-317
ISSN: 2052-2525