Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

Lee, H.-H.; Cherni, I.; Yu, H.; Fromme, R.; Doran, J.D.; Grotjohann, I.; Mittman, M.; Basu, S.; Deb, A.; Dörner, K.; Aquila, A.; Barty, A.; Boutet, S.; Chapman, H.N.; Doak, R.B.; Hunter, M.S.; James, D.; Kirian, R.A.; Kupitz, C.; Lawrence, R.M.; Liu, H.; Nass, K.; Schlichting, I.; Schmidt, K.E.; Seibert, M.M.; Shoeman, R.L.; Spence, J.C.H.; Stellato, F.; Weierstall, U.; Williams, G.J.; Yoon, C.; Wang, D.; Zatsepin, N.A.; Hogue, B.G.; Matoba, N.; Fromme, P.; Mor, T.S.

doi:10.1107/S2052252514014900

Figure 8
CTB^AAAAMPR is monodisperse as a high-order oligomer. (a) DLS measurements were performed so that the size distribution in the sample was analyzed for 20 s and the measurement was repeated consecutively ten times. The moment-to-moment fraction of particles estimated to have a particular hydrodynamic radius is color-coded and shown as a heat plot (red, >90%; blue, none). The narrow vertical and red profile shown indicates high stability over the duration of the measurement and low polydispersity. Time: the total duration of the scanning session (200 s). (b) A distribution curve of particle-size frequencies gives a more quantitative evaluation of the polydispersity, with the mean ± SD indicated next to the peak. The standard deviation of the size distribution is only 6% of the mean, indicating low polydispersity.

IUCrJ

Volume 1| Part 5| August 2014| Pages 305-317

ISSN: 2052-2525

doi:10.1107/S2052252514014900

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