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Figure 5
Structural characterization of RNA demethylases. (a) Cartoon representation of FTO in complex with m3T (PDB code: 3lfm ). The β-strands in the DSBH fold are labeled. Key features highlighted are NRL1 (dotted circle), NRL2 (dotted circle), βI–βII loop (blue) and CTD (cyan). (b) Cartoon representation of ALKBH5 in complex with 2OG (PDB code: 4oct ). The disulfide bond is shown in stick representation and labeled. (c) Close-up view of the ligand-binding sites in FTO. The conserved HxD/E…H…R…R motif and the ligands are shown in green and pale cyan, respectively. The metal ion is shown in sphere mode. The interactions are displayed as dashed lines. (d) Close-up view of the ligand-binding sites in ALKBH5. (e) Schematic of the FTO–citrate interaction (PDB code: 4ie7 ). The citrate is shown as stick and colored in blue. (f) Schematic of the ALKBH5–citrate interaction (PDB codes: 4o61 and 4nrm ). The citrate molecules from two structures are colored blue and pink, respectively.

IUCrJ
Volume 1| Part 6| October 2014| Pages 540-549
ISSN: 2052-2525
doi:10.1107/S2052252514020922