Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Nogly, P.; James, D.; Wang, D.; White, T.A.; Zatsepin, N.; Shilova, A.; Nelson, G.; Liu, H.; Johansson, L.; Heymann, M.; Jaeger, K.; Metz, M.; Wickstrand, C.; Wu, W.; Båth, P.; Berntsen, P.; Oberthuer, D.; Panneels, V.; Cherezov, V.; Chapman, H.; Schertler, G.; Neutze, R.; Spence, J.; Moraes, I.; Burghammer, M.; Standfuss, J.; Weierstall, U.

doi:10.1107/S2052252514026487

Figure 2
The experimental setup at the ID13 microfocus beamline. (a) (1) Microscope focused on the jet. (2) LCP injector with (3) nozzle close to the beamstop. (b) A view of the LCP nozzle as seen through the microscope. LCP was extruded towards the left as viewed in this projection, and the X-ray beam hits the stream at a distance of 40 µm from the end of the coned capillary. The capillary ID is 50 µm. A co-flowing gas stream (green arrows) keeps the LCP stream straight. (c) Schematic diagram of the setup. The water used to drive the injector is shown in blue, the LCP in red and the gas in green. (d) An SMX diffraction pattern from a bR microcrystal, with visible Bragg spots extending out to 2.2 Å resolution.

IUCrJ

Volume 2| Part 2| January 2015| Pages 168-176

ISSN: 2052-2525

doi:10.1107/S2052252514026487

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