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Figure 3
(Centre) Comparison of bR structures solved by SMX and conventional cryocrystallography (Cryo). The protein backbone of the room-temperature SMX structure (purple) superimposes well with the Cryo structure (blue). (Bottom) Retinal omit maps [blue (Cryo) or purple (SMX) mesh, 2Fo − Fc at 1.5σ; green mesh, Fo − Fc at 2.5σ] indicate increased flexibility in the β-ionone ring. The upper insets show a different rotamer for E194 involved in proton translocation, and indications for radiation damage on D38 exposed to the extramembrane environment.

IUCrJ
Volume 2| Part 2| January 2015| Pages 168-176
ISSN: 2052-2525