Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Nogly, P.; James, D.; Wang, D.; White, T.A.; Zatsepin, N.; Shilova, A.; Nelson, G.; Liu, H.; Johansson, L.; Heymann, M.; Jaeger, K.; Metz, M.; Wickstrand, C.; Wu, W.; Båth, P.; Berntsen, P.; Oberthuer, D.; Panneels, V.; Cherezov, V.; Chapman, H.; Schertler, G.; Neutze, R.; Spence, J.; Moraes, I.; Burghammer, M.; Standfuss, J.; Weierstall, U.

doi:10.1107/S2052252514026487

Figure 4
Cluster analysis of bR ground-state structures using hierarchical sorting. This analysis sorts according to the average of the absolute value of the difference between two internal distance matrices [S_jj(Å)] calculated on C^α atoms (Wickstrand et al., 2014

). PDB codes are given for all deposited wild-type structures of bR in its resting state. LCP bR structures are marked in purple. The room-temperature SMX structure (bRSMX) and the structure of bR solved here using conventional data collection and cryocooling (bRcryo) are marked in red. Inset: The internal distance matrix for bRSMX–bRcryo shows that cryocooling compresses helices A and B slightly towards helices D, E and F.

IUCrJ

Volume 2| Part 2| January 2015| Pages 168-176

ISSN: 2052-2525

doi:10.1107/S2052252514026487

Open

access

Follow IUCrJ

Search IUCr Journals		doi		Advanced search
Author		volume	page