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Figure 1
Diffraction pattern, electron density and structure of SERCA–Ca2–AMPPCP. (a) Representative diffraction pattern of SERCA–Ca2–AMPPCP microcrystals. The boxed inset shows a strong Bragg spot at 2.8 Å resolution {Miller index [31, −15, 24], 〈I/σ(I)〉 = 8.4}. (b) Left, overall 2mFo − DFc electron-density map (contoured at 1.0σ) after final refinement including coordinates and B factors for all atoms and data to 2.8 Å resolution; right, molecular model, including ten transmembrane helices and cytoplasmic N (nucleotide binding), P (phosphorylation) and A (actuator) domains. The 13 structural segments initially refined as rigid groups are shown in different colours. Ligand-binding regions are indicated by numbers: 1, AMPPCP; 2, Ca2+ ions; 3, lipid.

IUCrJ
Volume 2| Part 4| June 2015| Pages 409-420
ISSN: 2052-2525
doi:10.1107/S2052252515008969