A novel inert crystal delivery medium for serial femtosecond crystallography

Conrad, C.E.; Basu, S.; James, D.; Wang, D.; Schaffer, A.; Roy-Chowdhury, S.; Zatsepin, N.A.; Aquila, A.; Coe, J.; Gati, C.; Hunter, M.S.; Koglin, J.E.; Kupitz, C.; Nelson, G.; Subramanian, G.; White, T.A.; Zhao, Y.; Zook, J.; Boutet, S.; Cherezov, V.; Spence, J.C.H.; Fromme, R.; Weierstall, U.; Fromme, P.

doi:10.1107/S2052252515009811

Figure 1
Diagram showing how the crystals are embedded into the agarose medium. (a) A dense pellet of crystals is drawn up into a syringe, (b) the agarose solution (contained in a 15 ml centrifuge tube) is submerged in boiling water until the agarose dissolves, the liquid agarose is drawn up into a warmed syringe and the agarose is allowed to gel and equilibrate to room temperature, (c) the protein crystals and agarose syringe are connected by a syringe coupler and (d) using the syringe coupler, the crystals are embedded throughout the agarose by moving the plungers back and forth.

IUCrJ

Volume 2| Part 4| June 2015| Pages 421-430

ISSN: 2052-2525

doi:10.1107/S2052252515009811

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