view article

Figure 1
Diagram showing how the crystals are embedded into the agarose medium. (a) A dense pellet of crystals is drawn up into a syringe, (b) the agarose solution (contained in a 15 ml centrifuge tube) is submerged in boiling water until the agarose dissolves, the liquid agarose is drawn up into a warmed syringe and the agarose is allowed to gel and equilibrate to room temperature, (c) the protein crystals and agarose syringe are connected by a syringe coupler and (d) using the syringe coupler, the crystals are embedded throughout the agarose by moving the plungers back and forth.

Volume 2| Part 4| June 2015| Pages 421-430
ISSN: 2052-2525