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Figure 1
Examination of the cell morphology of rapidly frozen N. caninum with cryo-protectants. Extracellular N. caninum tachyzoites were resuspended in a tissue culture medium (a), cryo-protectants only (b), and cryo-protectants followed by rapid freezing in liquid nitrogen (c). The cells were imaged with an AxioCam MRm CCD camera and AxioVision software on an Axio Imager Z1 microscope (Zeiss) using a 100× oil immersion objective. Rapidly frozen tachyzoites with cryo-protectants exhibited similar morphology to those parasites that were resuspended in cryo-protectants only and in a tissue culture medium. Scale bar: 5 µm.

Volume 2| Part 5| September 2015| Pages 575-583
ISSN: 2052-2525