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Figure 2
Schematic layout of a three-dimensional cryo-CDI microscope. (a) Diagram showing a silicon nitride membrane containing frozen-hydrated cells on a single-tilt piezo-electric stage, bathed in a nitrogen gas cryostream. A coherent X-ray beam impinges on a cell, from which a representative tilt series of 72 diffraction patterns was measured using a MAXIPIX detector (b). A three-dimensional diffraction pattern was assembled from the 72 two-dimensional diffraction patterns, from which the three-dimensional structure of the cell (c) was iteratively reconstructed by using the oversampling smoothness (OSS) algorithm (Rodriguez et al., 2013BB32).

Volume 2| Part 5| September 2015| Pages 575-583
ISSN: 2052-2525