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Figure 1
(a) Electron micrograph of a wt Hp cell containing crystalline alcohol oxidase (AO) in electron-dense peroxisomes (P) seen next to mitochondria (M) and a vacuole (V). The crystalline matrix is visible in the regular striated pattern observed at higher magnification (b). Also note the single membrane outlining the organelle and enclosing the crystal. (c) Schematic representation of peroxisome proliferation. Deletion of the cytosolic peroxisomal cargo receptor Pex5, which is also part of the peroxisomal translocon, prevents import of AO into the peroxisomal matrix and results in cytosolic AO crystals. (d, e) ΔPEX11 cells display compromised fission and result in fewer (typically one) and larger peroxisomes per cell as observed by fluorescence microscopy with the peroxisomal membrane label Pmp47-mGFP. Scale bars are 2 µm in length. (f) Mean radius distributions from dynamic light scattering for purified fractions of wt (black) and ΔPEX11 (red) peroxisomes.

Volume 3| Part 2| March 2016| Pages 88-95
ISSN: 2052-2525