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Figure 4
Solution structure of TcCRT: full-length molecule and crystallized construct. (a) The comparison between the theoretical (CRYSOL) and experimental scattering curves of the crystallized TcCRT construct shows a difference in the Porod domain, which suggests that the relative orientation of the domains is different in the solution structure compared with the X-ray structure. (b) Different orientations of the C-­terminal helix proposed by BUNCH improved the fit (c) in this case. The ten best models are shown here (with χ2 values between 0.96 and 1.03). (d, e) In the case of the full-length TcCRT solution structure, combinations of C-terminal and P-domain reorientations with BUNCH improve the curve fit to the Porod domain (0.3 nm). The ten best results are shown here (with χ2 values between 3.95 and 5.34). Local predictions for undefined segments in the initial protein models are shown by dots in (b) and (d).

IUCrJ
Volume 3| Part 6| November 2016| Pages 408-419
ISSN: 2052-2525
https://doi.org/10.1107/S2052252516012847