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Figure 4
CADEE workflow. Basic input files are supplied to CADEE (1) and initial screening mutagenesis such as a computational alanine scan is performed (2). Automated analysis of the results is then performed and the user chooses which mutation hotspots should be mutated to which library (3). The next round of in silico evolution is then started (4) and automatically analyzed (5). Depending on the user input, (6) another round of evolution is performed (3–6) or the process is stopped (7) if the results are considered satisfactory.

IUCrJ
Volume 4| Part 1| January 2017| Pages 50-64
ISSN: 2052-2525
https://doi.org/10.1107/S2052252516018017