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Figure 2
Nuclear density map showing protonation of His57 in trypsin. Left, difference density map showing protonation of Nδ of His57. Center, 2FoFc nuclear density map with double protonation of His57 and negative density peaks for the unexchanged H atoms. Right, difference density map with the proton modeled on Asp102. The contour shows that the proton belongs on His57 (solid lines) and not Asp102 (dotted lines). Reprinted with permission from Kossiakoff & Spencer (1981BB48), Biochemistry, 20, 6462–6474. Copyright (1981) American Chemical Society.

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