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Figure 1
Overview of mass spectrometry-based phosphoproteomic workflows. Depending on the experimental design, there are a number of different strategies which can be chosen to enrich specific compartments of the phosphoproteome. This is commonly followed by fractionation to reduce complexity and increase coverage of complex cell lysates. Finally, the method of data acquisition will be influenced by the specific biological questions of interest when interrogating proteomic data.

IUCrJ
Volume 4| Part 2| March 2017| Pages 119-130
ISSN: 2052-2525
https://doi.org/10.1107/S2052252516020546