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Figure 4
Schematic drawing of data-collection strategies and each change of diffraction intensity from a single crystal using a microbeam. The total image number in single-point data collection is limited by radiation damage. Multi-point data collection can increase the total image number by avoiding serious radiation damage by using a fresh crystal volume with translation of the irradiation point. Helical data collection enables an equal distribution of radiation damage over the entire crystal volume. Thus, the helical method gives completely smoothed scales and B factors for frames, which finally improves the data quality.

IUCrJ
Volume 4| Part 5| September 2017| Pages 529-539
ISSN: 2052-2525
https://doi.org/10.1107/S2052252517008193