Figure 10
Effect of ABC optimization. After ABC alignment of the full data set, all images are rotated and shifted to a common origin as dictated by the Euler orientations of the class averages. A new MSA classification thus yields a better class average since more molecular images now have a common three-dimensional orientation. Shown here are class averages at an intermediate level of the refinement procedures at a coarsening level of 2 (Nyquist frequency 1/4.4 Å). |