Figure 11
FSC curves of the final three-dimensional reconstruction. (0.6 of the Nyquist frequency corresponds to 1/3.7 Å.) FSCβ<60° is based on the preferred top-view class averages (0 < β < 60°); FSCβ>60° assesses the contributions of the side-view class averages (60 < β < 90°). The circle indicates the area in which a high FSC value is a good indicator of the quality of the reconstruction. |