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Figure 7
15 class averages of unaligned particles (`alignment by classification'; ABC) used for an initial `random-startup' three-dimensional reconstruction. Class average 2 corresponds to a top view and class averages 3 and 10 correspond to side views. The other class averages represent various intermediate projection images of the haemoglobin. Since the input images are coarsened (binned) by a factor of four, no information beyond the Nyquist frequency of 1/8.8 Å can be present in the images at this stage.

IUCrJ
Volume 4| Part 5| September 2017| Pages 678-694
ISSN: 2052-2525
https://doi.org/10.1107/S2052252517010922