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Figure 1
Cryo-EM structure determination of the chlororibosome to 3.2 Å resolution within 24 h. The experiment started with seven spinach leaves and chlororibosomes were purified within 5 h, appearing as a major peak in the sucrose gradient. Contaminating fractions labelled with asterisks were included for grid preparation. Data collection started after 7 h, and micrographs exhibited ice contamination owing to high humidity conditions during grid handling. The data were processed on-the-fly, resulting in a 3.2 Å resolution reconstruction after 24 h.

Volume 4| Part 6| November 2017| Pages 723-727
ISSN: 2052-2525