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Figure 1
Overall structure and oligomeric interfaces of VvPrx3. (a) Ribbon diagram of the reduced Prx3 (C48D/C73S) protomer; α-helices, β-strands and loops are represented in gold, green and grey, respectively. The black arrows indicate the side chains of the mutated residues Cys48 and Cys73. (b) Dimeric structure of reduced Prx3 (C48D/C73S) formed by an A-type interface (black circle). The two protomers are coloured differently (green and salmon). The side chains of Cys48 and Cys73 are shown in ball-and-stick representation. The black arrows indicate the side chains of the mutated residues (C48D). (c) The asymmetric unit of oxidized Prx3 (C73S) with intact Cys48. Two Cys48 residues from adjacent protomers form a disulfide bond. The crystals of oxidized Prx3 (C73S) were grown without reducing agent. Four protomers are drawn in different colours and the side chains of Cys48 and Ser73 are displayed as ball-and-stick models. The red circle represents the C-type interface.

IUCrJ
Volume 5| Part 1| January 2018| Pages 82-92
ISSN: 2052-2525
https://doi.org/10.1107/S205225251701750X